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Test Code FRAG Osmotic Fragility, Erythrocytes


Advisory Information


 



Additional Testing Requirements


 



Shipping Instructions


Specimens must arrive within 72 hours of collection.



Necessary Information


Patient's age and sex are required.



Specimen Required


Both a whole blood EDTA specimen and a control specimen are required as temperature extremes can increase the fragility of the specimen and cause false-positive results.

 

Patient:

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Immediately refrigerate specimen after collection. Refrigerate at 0 to 4° C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.

2. Send specimen in original tube. Do not aliquot.

3. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.

4. Be sure specimen and control are stored and transported together at refrigerated temperature, carefully following proper handling and shipping instructions.

 

Normal Shipping Control:

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.

2. Handwrite "normal control" clearly on the outermost label.

3. Immediately refrigerate specimen after collection. Refrigerate at 0 to 4° C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.

4. Send specimen in original tube. Do not aliquot.

5. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.


Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.

Useful For

Evaluation of suspected hereditary spherocytosis associated hemolytic anemia

 

Confirming or detecting mild spherocytosis

Profile Information

Test ID Reporting Name Available Separately Always Performed
FRAGO Osmotic Fragility No Yes
SCTRL Shipping Control Vial No Yes

Method Name

Osmotic Lysis

Reporting Name

Osmotic Fragility, RBC

Specimen Type

Control
Whole Blood EDTA

Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Control Refrigerated 72 hours PURPLE OR PINK TOP/EDTA
Whole Blood EDTA Refrigerated 72 hours

Reject Due To

Gross hemolysis Reject
Clotted blood Reject

Reference Values

≥12 months:

0.50 g/dL NaCl (unincubated): 3-53% hemolysis

0.60 g/dL NaCl (incubated): 14-74% hemolysis

0.65 g/dL NaCl (incubated): 4-40% hemolysis

0.75 g/dL NaCl (incubated): 1-11% hemolysis

 

Reference values have not been established for patients who are <12 months of age.

Day(s) and Time(s) Performed

Monday through Saturday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

85557

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FRAG Osmotic Fragility, RBC In Process

 

Result ID Test Result Name Result LOINC Value
9064 Osmotic Fragility, RBC 34964-7
SCTRL Shipping Control Vial 40431-9
3306 Osmotic Fragility, 0.50 g/dL NaCl 23915-2
3307 Osmotic Fragility, 0.60 g/dL NaCl 23918-6
3308 Osmotic Fragility, 0.65 g/dL NaCl 23920-2
3309 Osmotic Fragility, 0.75 g/dL NaCl 23921-0
3310 Osmotic Fragility Comment 59466-3