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Test Code FRAG Osmotic Fragility, Erythrocytes


Ordering Guidance


 



Additional Testing Requirements


 



Shipping Instructions


Specimens must arrive within 72 hours of collection.



Necessary Information


Patient's age is required.



Specimen Required


Both a whole blood EDTA specimen and a shipping control specimen are required. The shipping control specimen is used to evaluate whether a patient result has been compromised by handling conditions such as temperature, motion, or other transportation interferences. Temperature and handling extremes can adversely impact the integrity of the specimen.

 

Patient:

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Refrigerate specimen immediately after collection.

2. Send whole blood specimen in original tube. Do not aliquot.

3. Rubber band patient specimen and control vial together.

 

Normal Shipping Control:

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Collect a shipping control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.

2. Clearly hand write “normal control” on the outermost label.

3. Refrigerate specimen immediately after collection.

4. Send control specimen in original tube. Do not aliquot.

5. Rubber band patient specimen and control vial together. The control and patient specimen must be handled in the same manner from specimen collection to receipt in the testing laboratory.


Orlando Health Note:

** Specimen must be sent to the laboratory on ice**

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.

Useful For

Evaluating suspected hereditary spherocytosis-associated hemolytic anemia

 

Confirming or detecting mild spherocytosis

Profile Information

Test ID Reporting Name Available Separately Always Performed
FRAGO Osmotic Fragility No Yes
SCTRL Shipping Control Vial No Yes

Method Name

Osmotic Lysis

Reporting Name

Osmotic Fragility, RBC

Specimen Type

Control
Whole Blood EDTA

Specimen Minimum Volume

Patient whole blood, shipping control: 2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Control Refrigerated 72 hours PURPLE OR PINK TOP/EDTA
Whole Blood EDTA Refrigerated 72 hours

Reject Due To

Gross hemolysis Reject
Clotted blood Reject

Reference Values

≥12 months:

0.50 g/dL NaCl (unincubated): 3-53% hemolysis

0.60 g/dL NaCl (incubated): 14-74% hemolysis

0.65 g/dL NaCl (incubated): 4-40% hemolysis

0.75 g/dL NaCl (incubated): 1-11% hemolysis

 

NaCl = sodium chloride

Reference values have not been established for patients who are younger than 12 months of age.

Day(s) Performed

Monday through Saturday

Report Available

2 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85557

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FRAG Osmotic Fragility, RBC 98904-6

 

Result ID Test Result Name Result LOINC Value
9064 Osmotic Fragility, RBC 34964-7
SCTRL Shipping Control Vial 40431-9
3306 Osmotic Fragility, 0.50 g/dL NaCl 23915-2
3307 Osmotic Fragility, 0.60 g/dL NaCl 23918-6
3308 Osmotic Fragility, 0.65 g/dL NaCl 23920-2
3309 Osmotic Fragility, 0.75 g/dL NaCl 23921-0
3310 Osmotic Fragility Comment 59466-3

Secondary ID

9064