Sign in →

Test Code MTDNU Methadone Confirmation, Random, Urine

Ordering Guidance

1. For situations where chain of custody is required, a Chain-of-Custody Kit (T282) is available. For chain-of-custody testing, order MTDNX / Methadone Confirmation, Chain of Custody, Random, Urine.

2. Additional drug panels and specific requests are available. Call 800-533-1710 or 507-266-5700.

3. If urine creatinine is required or adulteration of the sample is suspected, order ADULT / Adulterants Survey, Random, Urine.

Specimen Required

Supplies: Urine Tubes, 10 mL (T068)

Container/Tube: Plastic, 10-mL urine tube

Specimen Volume: 10 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.

Useful For

Monitoring for compliance of methadone treatment for analgesia or drug rehabilitation


Assessing compliance with rehabilitation programs by urine measurement of 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine

Method Name

Gas Chromatography Mass Spectrometry (GC-MS) Confirmation with Quantitation

Reporting Name

Methadone Confirmation, U

Specimen Type


Specimen Minimum Volume

2.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  14 days

Reject Due To

Gross hemolysis OK
Gross icterus OK

Reference Values



Cutoff concentrations:


<100 ng/mL


<100 ng/mL

Day(s) Performed

Tuesday, Thursday

Report Available

3 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MTDNU Methadone Confirmation, U In Process


Result ID Test Result Name Result LOINC Value
56028 EDDP-by GC-MS 58428-4
83129 Methadone-by GC-MS 16246-1
21107 Methadone Interpretation 69050-3
21110 Chain of Custody 77202-0

Secondary ID



If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.