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HelpTest Code CDSP Celiac Disease Serology Cascade, IgA/IgG with Reflex to HLA-DQ Typing
Department
Chemistry/ Immunology
Useful For
Evaluating patients suspected of having celiac disease, including patients with compatible symptoms, patients with atypical symptoms, and individuals at increased risk (family history, previous diagnosis with associated disease)
Comprehensive algorithmic evaluation including human leukocyte antigen typing
Specimen Requirements
Both blood and serum are required.
Specimen Type: Blood
Container/Tube: Yellow top (ACD solution B)
Specimen Volume: 6 mL
Collection Instructions: Send whole blood in original tube. Do not aliquot.
Specimen Type: Serum
Collection Container/Tube: Gold Top SST
Preferred: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 2 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Testing Algorithm
If the IgA result is within the age-specified normal range, then tissue transglutaminase (tTG) IgA antibody will be performed at an additional charge.
If tTG IgA antibody result is equivocal, then endomysial IgA antibodies and deamidated gliadin IgA antibody testing will be performed at an additional charge.
If IgA is greater than or equal to 0.5 mg/dL but lower than age-specified normal, then tTG IgA, tTG IgG, deamidated gliadin IgA, and deamidated gliadin IgG antibody testing will be performed at an additional charge.
If IgA is below the limit of detection (<0.5 mg/dL), then tTG IgG and deamidated gliadin IgG antibody testing will be performed at an additional charge.
For more information see Celiac Disease Serology Cascade with Reflex Algorithm
Ship Temperature
Room Temperature
Stability
Serum
Room Temperature - 8 Hours
Refrigerated - 2 weeks
Frozen -20C - > 2 weeks
Method
IgA: Nephelometry
Tissue Transglutaminase and Gliadin (Deamidated): ELISA
Day(s) Performed
Monday to Friday, 7:00 am - 3:00pm
CPT Code
81376
86258 (if appropriate)
86364 (if appropriate)
Group Components
IgA
Cascade reflexes to the appropriate test in the algorithm.
Tissue Transglutaminase IgA
Tissue Transglutaminase IgG
Gliadin (Deamidated) IgA
Gliadin (Deamidated) IgG